THE TITRIMETRIC DETERMINATION OF “LACTOBACILLUS CASEI FACTOR” AND “FOLIC ACID”
نویسندگان
چکیده
منابع مشابه
The Titrimetric Determination of “lactobacillus Case1 Factor” and “folic Acid”*
Microbiological tests have aided greatly in the isolation work on “L&o!x~ciZZus casei factor,” demonstrated by Snell and Peterson in 1940 (l), and “folic acid,” the nutrient required by Streptococcus lactis R as defined by Mitchell et al. (2). It seems likely that bacterial assays will continue to play an important role in the study of these compounds, since development of chemical methods has ...
متن کاملThe activity of Lactobacillus casei factor, folic acid, and vitamin Bc for Streptococcus faecalis and Lactobacillus casei.
It has been established that the terms vitamin M, vitamin B,, norit eluate factor, Lactobacillus casei factor, factor U, folic acid, and factor SLR all refer to a group of compounds (or a compound) with similar characteristics and similar biological activities. Of these factors seven have been reported in purified form: folic acid (isolated from spinach) (l), three L. casei factors (one from li...
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Folate-depleted, cryoprotected preparations of Lactobacillus casei are stable for at least eight months at -18 degrees C, capable of reproducible growth and suitable as a ready source of inoculum for measurement of folates in physiological fluids. Cryoprotected microorganisms can be a commercially available laboratory reagent, thus simplifying and further standardizing the microbiological assay...
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A simple procedure for preparing glycerol-cryoprotected Lactobacillus casei cultures has been developed. L. casei grown in medium supplemented with low concentrations of folic acid (0.3 micrograms/L) is diluted with an equal volume of glycerol (800 mL/L) and stored at -20 degrees C. Growth response of the glycerol-cryoprotected L. casei to low concentrations of folic acid exceeded that of cult...
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[8H]Methotrexate was metabolized mainly to 4-amino-4deoxy-lo-methylpteroic acid in resting cells of ZactobaciZZus casei and a small amount of methotrexate remained inside the cells after 24 hours of incubation. Preincubation of L. casei with methotrexate inhibited the incorporation of [3H]folic acid completely and I-5-formyl-[3H]tetrahydrofolic acid partially into pteroyloctaglutamate and incre...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1945
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)41651-6